News

Preparation of select murine brain sections for HPF, cryo-CLEM, and cryo-ET

July 29, 2021 Michael H.B. Stowell, CCET Join us and learn about the NIH-funded National Network for Cryo-Electron Tomography Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. Michael H.B. Stowell of the CU Boulder Center for Cryo Electron Tomography will present on preparing murine brain sections with HPF, cryo-CLEM, and cryo-FIB techniques. The webinar provides a background on the isolation of tissues, embedding in agar, vibratome sectioning, and use of high-pressure freezing techniques. Register to attend at  https://go.wisc.edu/5p84g1 Recordings at https://www.cryoetportal.org/webinar_series/

Correlative cryogenic light and electron microscopy and tomography

July 22, 2021 Gong-Her Wu, SCSC Join us and learn about the NIH-funded National Network for Cryo-Electron Tomography Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. Gong-Her Wu presents an overview of the imaging technologies and resolution ranges. This talk covers workflows for multi-scale cryo-correlative imaging of cells including confocal microscopy, cryo-EM, and cryo-FIB-SEM. Register to attend at  https://go.wisc.edu/5p84g1 Recordings at https://www.cryoetportal.org/webinar_series/

High pressure freezing, the waffle method and cryo-FIB

July 15, 2021 Mykhailo Kopylov, NCITU Join us and learn about the NIH-funded National Network for Cryo-Electron Tomography Mykhailo Kopylov from the National Center for In-situ Tomographic Ultramicroscopy (NCITU) presents on the High-Pressure Freezing "waffle" method for vitrification of samples including mammalian cells, cell clumps like bacteria or yeasts, purified proteins, large cells and cell aggregates, and tissues with 10 um limit. He provides an overview of the problems for vitrification of samples and how the HPF waffle technique provides new sample workflows. Register to attend at  https://go.wisc.edu/5p84g1 Recordings at https://www.cryoetportal.org/webinar_series/

An introduction to cryo-ET and overview of the National Network for Cryo-ET

July 8, 2021 Elizabeth R. Wright, MCCET Join us and learn about the NIH-funded National Network for Cryo-Electron Tomography  Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. The National Network for Cryo-Electron Tomography is an NIH-funded consortium whose mission is to provide access to both the instrumentation and expert staff for advancing the original research of scientific investigators and for training those new to cryo-electron tomography. Join us in this webinar series to learn more about this network and about how you can access these world-class cryo-electron tomography resources in your research program. Register to attend at  https://go.wisc.edu/5p84g1 [...]

NIH Funds National Network for Cryoelectron Tomography

The NIH Common Fund’s Transformative High Resolution Cryoelectron Microscopy (CryoEM) Program has funded the National Network for Cryoelectron Tomography (cryoET) as part of its initiative to advance the application of cryoET. This network will offer the biomedical community access to a specialized cryoelectron microscopy (cryoEM) technique that is uniquely capable of visualizing intact regions of cells and tissues at high resolution and with little perturbation.

The National Network for CryoET will be established with four service centers, including one service center that will also serve as the central network hub. The network hub will be located at University of Wisconsin-Madison and led by Dr. Elizabeth Wright. The network service centers will be located at the New York Structural Biology Center (Dr. Bridget Carragher, contact), SLAC National Accelerator Laboratory-Stanford University (Dr. Wah Chiu), and University of Colorado Boulder (Dr. Andreas Hoenger, contact). The service centers will specialize in cryoET specimen preparation. The hub will perform cryoET data collection for all the user laboratories served by the network, in addition to its service center functions.

Aquilos 2 is ordered!

Estimated delivery time – 1st quarter 2021

Leica GP2 is here!

Side-blotting plunge-freezer dedicated to cell culture work is installed in a temporary NCITU BSL-2 area at SEMC.

Construction is started on a NCITU laboratory space

Estimated completion Q4 2020